qcrB [2018-07-03 10:45:24]

menaquinol:cytochrome c oxidoreductase (cytochrome b subunit), component of the cytochrome bc complex

locus: BSU22550

pI: 7.44

mw: 25.34 kDa

protein length: 224 aa

gene length: 672 bp

function: respiration

product: menaquinol:cytochrome c oxidoreductase (cytochrome b subunit)

essential: no

synonyms: bfcB,petB

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.2|Respiration] → [category|SW 2.1.2.4|Respiration/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,363,913 → 2,364,587

The protein

Protein family

• peptidase M20A family (according to Swiss-Prot)

[SW|Cofactors]

• heme, covalently bound to Cys-43 [pubmed|9535866]

Structure

• [PDB|1VFS] (43% identity)

[SW|Localization]

• cell membrane [Pubmed|23880299]

Expression and Regulation

Operons

• genes: [gene|D4AF96C936093BA44E46FF92A3EDE437495B0923|qcrA]-[gene|1DC5C6DAF0AB1EB59BC9361DC1161E75F99AFE33|qcrB]-[gene|F2973C512AFB4B9F8FE0CCAF09646E60F886C013|qcrC]
  description: [Pubmed|7592464]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7592464], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]: activation, [Pubmed|8631715], in [regulon|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|12850135], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • induced by oxygen limitation ([protein|search|ResD]) [Pubmed|8631715]

Biological materials

Mutant

• BKE22550 (Δ[gene|1DC5C6DAF0AB1EB59BC9361DC1161E75F99AFE33|qcrB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCAGTCATAAATTTTGTTCA, downstream forward: _UP4_TAAACTCTTGATCTGCGAAT
• BKK22550 (Δ[gene|1DC5C6DAF0AB1EB59BC9361DC1161E75F99AFE33|qcrB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCAGTCATAAATTTTGTTCA, downstream forward: _UP4_TAAACTCTTGATCTGCGAAT

References

• 8631715,7592464,12107147,12850135,20817675,23880299,9535866,27503613